Te whakateretere i te whakarite pūtau CAR-T hei whakapai ake i ngā putanga mō te tūroro

Te rongoā pūtau CAR-T, he maimoatanga hou mō Te mate pukupuku toto o te pūtau-B me te lymphoma, kei te piki haere te tūhuratanga mō ētahi atu mate pukupuku toto, ngā pukupuku totoka, me ngā mate pūnaha ārai mate. Heoi, ko te whakawhānui ake i tēnei rongoā ki ngā tohu hou he wero whakahaere, nā te mea ka roa pea te hanga pūtau CAR-T o nāianei ki te 3-6 wiki. He arotake tata nei i roto iTe Rongoā Matepukupuku Lancet e matapakihia ana ngā whanaketanga nui o te hanga pūtau CAR-T e whai ana ki te whakahaere i ngā tukanga, te whakarei ake i te kounga o ngā pūtau, me te whakawhānui ake i te urunga atu o ngā tūroro.

I ngā wā o mua, ko te whakarite pūtau CAR-T me nui te whakahohenga me te whakawhānui i ngā pūtau in vitro, ka taea ai e tēnei te arahi ki te ngenge o ngā pūtau me te whakaiti i te whai huatanga o te rongoā. Ko ngā mahi auaha hou, pēnei i ngā tikanga whakawhānui-iti, ngā huarahi kore-whakahohenga, me te whakaputanga i ngā wāhi tiaki, ka tere ake te hurihanga me te iti o te whakahaere in vitro. Ko ēnei tikanga, kei te whakatinanahia inaianei i roto i ngā whakamātautau haumanu me ngā whakamātautau arumoni. Ngā Hua CAR-T, e whakanui ana i te hanga o ngā pūtau CAR-T kāore i te tino rerekē te āhua, ā, e ātete ana ki te ngenge, ka hua ake he maimoatanga ka roa ake te mau tonu mō te ārai i te pukupuku.

E whakaatu ana hoki te arotake i te ahunga whakamua o te kōwhiringa pūtau-T i mua i te tango i ngā pūtau kāore e hiahiatia, ngā pūtau ārai mate rānei, e whakarei ake ana i te kaha rongoā o ngā hua CAR-T. Ko ngā pūnaha e arotau ana i te wā leukapheresis i runga i ngā hua toto i mua i te maimoatanga ka whakapai ake i te whai huatanga me te urunga atu o ngā rauemi.

Kei te whakaatu kē ngā tikanga whakarite tere o te CAR-T i ngā hua whai hua. E whakaatu ana ngā whakamātautau haumanu ka taea e ngā wā hanga poto ake te whakapai ake i te pumau me te whai huatanga o ngā pūtau, me ngā rangahau tonu e tūhura ana i ngā tikanga hanga tino tere e kawe mai ana i ngā pūtau CAR-T ki ngā tūroro i roto i te rā kotahi.

Haunga ngā tikanga whakawhānui-iti, kei te whakanui ake te hangarau pūtau auaha i te whai huatanga o te CAR-T. Kei roto i ngā tikanga te whakamahinga o ngā aukati ngota iti i te wā e hangaia ana hei tiaki i ngā momo mahara pūtau-T me te ātete ki te ngenge. Hei tauira, kei te whakauruhia ngā aukati AKT ki roto i ngā kawa whakaputa CAR-T, e whakaatu ana i te kaha ārai-pukupuku kua pai ake i roto i ngā tauira leukemia preclinical. Heoi anō, kei te whakaatu ngā pūtau CAR-T i hangaia hei huna i ngā cytokines immunostimulatory, pērā i te IL-18, i te pai ake o te whakahaere pukupuku in vivo me te tipu pūtau tonu i roto i ngā whakamātautau haumanu wā-tōmua.

I te tipu haere o te hiahia o te ao mō ngā maimoatanga CAR-T, ka puta mai i ēnei whanaketanga te kaha ki te whakarerekē i te tiaki tūroro, kia tere ake ai te uru atu ki te maimoatanga whai hua, kia whānui ake ai hoki.