Leano la Mohlohlelletsi ea Ncha le Ntlafatsa Polokeho le Katleho ea Kalafo ea CAR-T ho Leukemia e Hlobaetsang ea Lisele tsa B
Beijing, Chaena – Phupu 23, 2024 – Nts'etsopele e ncha, Sepetlele sa Lu Daopei, ka tšebelisano 'moho le Hebei Senlang Biotechnology, se senotse liphetho tse tšepisang ho tsoa phuputsong ea bona ea morao-rao mabapi le kalafo ea sele ea chimeric antigen receptor T (CAR-T). Phuputso ena, e shebaneng le katleho le polokeho ea lisele tsa CAR-T tse entsoeng ka li-promoter tse fapaneng, e tšoaea tsoelo-pele e kholo kalafong ea leukemia ea B-ALL e khutlileng kapa e sa sebetseng hantle.
Phuputso ena, e nang le sehlooho se reng "Tšebeliso ea Promoter e Laolang Botenya ba Limolek'hule tsa CAR e ka Fetola Kinetics ea Lisele tsa CAR-T In Vivo," e hlahloba kamoo khetho ea promoter e ka susumetsang ts'ebetso ea lisele tsa CAR-T. Bafuputsi Jin-Yuan Ho, Lin Wang, Ying Liu, Min Ba, Junfang Yang, Xian Zhang, Dandan Chen, Peihua Lu, le Jianqiang Li ba tsoang Hebei Senlang Biotechnology le Lu Daopei Hospital ba etelletse pele lipatlisiso tsena.
Liphuputso tsa bona li bontša hore ho sebelisa MND (myeloproliferative sarcoma virus MPSV enhancer, negative control region NCR deletion, d1587rev primer binding site replacement) promoter liseleng tsa CAR-T ho lebisa ho density e tlase ea limolek'hule tsa CAR, e leng se fokotsang tlhahiso ea cytokine. Sena se bohlokoa hobane se thusa ho fokotsa litla-morao tse mpe tse atisang ho amahanngoa le phekolo ea CAR-T, joalo ka cytokine release syndrome (CRS) le CAR-T cell-related encephalopathy syndrome (CRES).
Teko ea bongaka, e ngolisitsoeng tlas'a sesupo sa ClinicalTrials.gov NCT03840317, e ne e kenyelletsa bakuli ba 14 ba arotsoeng ka lihlopha tse peli: e 'ngoe e amohelang lisele tsa CAR-T tse tsamaisoang ke MND le e 'ngoe e amohelang lisele tsa CAR-T tse tsamaisoang ke promoter ea EF1A. Hoa makatsa hore ebe bakuli bohle ba phekoloang ka lisele tsa CAR-T tse tsamaisoang ke MND ba fihletse phomolo e felletseng, 'me boholo ba bona ba bontša boemo bo fokolang ba ho se be le lefu kamora khoeli ea pele. Phuputso e boetse e tlalehile palo e tlase ea CRS le CRES e matla ho bakuli ba phekoloang ka lisele tsa CAR-T tse tsamaisoang ke MND ha li bapisoa le tse phekoloang ka lisele tse tsamaisoang ke EF1A.
Ngaka Peihua Lu oa Sepetlele sa Lu Daopei o bontšitse tšepo mabapi le bokhoni ba mokhoa ona o mocha, a re, "Tšebelisano-'moho ea rona le Hebei Senlang Biotechnology e hlahisitse temohisiso ea bohlokoa mabapi le ho ntlafatsa phekolo ea lisele tsa CAR-T. Ka ho fetola promoter, re ka ntlafatsa boemo ba polokeho ba phekolo ha re ntse re boloka katleho ea eona. Ena ke mohato oa bohlokoa oa ho etsa hore phekolo ea CAR-T e fumanehe habonolo le hore e mamellehe bakeng sa bakuli."
Phuputso ena e tšehelitsoe ke lithuso tse tsoang ho Natural Science Foundation ea Profense ea Hebei le Lefapha la Saense le Theknoloji la Profense ea Hebei. E totobatsa bohlokoa ba khetho ea batšehetsi nts'etsopele ea mekhoa ea phekolo ea lisele tsa CAR-T 'me e bula litsela tse ncha bakeng sa polokeho le katleho e kholoanyane. Kalafo ea Kankeres.