Icebo Lokukhuthaza Elitsha Liphucula Ukhuseleko kunye Nokusebenza Kakuhle Konyango lwe-CAR-T kwi-Acute B Cell Leukemia
EBeijing, eTshayina – Julayi 23, 2024 – Kwiphulo elimangalisayo, iSibhedlele iLu Daopei, ngokubambisana neHebei Senlang Biotechnology, sityhile iziphumo ezithembisayo ezivela kuphando lwaso lwamva nje malunga nonyango lweeseli ze-chimeric antigen receptor T (CAR-T). Olu phononongo, olugxile ekusebenzeni kakuhle nasekukhuselekeni kweeseli ze-CAR-T ezenziwe ngeepromoter ezahlukeneyo, lubonisa inkqubela phambili ebalulekileyo kunyango lwe-relapse okanye refractory acute B cell leukemia (B-ALL).
Olu phononongo, olunesihloko esithi "Ukusetyenziswa kwePromoter Regulating the Surface Density of CAR Molecules May Modulate the Kinetics of CAR-T Cells In Vivo," luhlola indlela ukhetho lwepromoter olunokuchaphazela ngayo ukusebenza kweeseli zeCAR-T. Abaphandi uJin-Yuan Ho, uLin Wang, uYing Liu, uMin Ba, uJunfang Yang, uXian Zhang, uDandan Chen, uPeihua Lu, kunye noJianqiang Li abavela kwiHebei Senlang Biotechnology kunye neLu Daopei Hospital bakhokela olu phando.
Iziphumo zabo zibonisa ukuba ukusebenzisa i-MND (myeloproliferative sarcoma virus MPSV enhancer, negative control region NCR deletion, d1587rev primer binding site replacement) promoter kwiiseli ze-CAR-T kukhokelela ekuxineni okuphantsi komphezulu weemolekyuli ze-CAR, nto leyo enciphisa imveliso ye-cytokine. Oku kubalulekile kuba kunceda ekunciphiseni iziphumo ebezingalindelekanga ezidla ngokunxulunyaniswa nonyango lwe-CAR-T, ezifana ne-cytokine release syndrome (CRS) kunye ne-CAR-T cell-related encephalopathy syndrome (CRES).
Uvavanyo lweklinikhi, olubhaliswe phantsi kwesazisi seClinicalTrials.gov i-NCT03840317, luquka izigulane ezili-14 ezahlulwe zaba ngamaqela amabini: elinye elafumana iiseli zeCAR-T eziqhutywa yi-MND kunye nelinye elafumana iiseli zeCAR-T eziqhutywa yi-EF1A. Okumangalisayo kukuba, zonke izigulane ezanyangwa ngeeseli zeCAR-T eziqhutywa yi-MND zifumene uxolelo olupheleleyo, uninzi lwazo lubonisa imeko encinci yokungafumani zifo emva kwenyanga yokuqala. Olu phononongo lukwabike ukuba kukho ukuhla kwenani le-CRS kunye ne-CRES ezinzima kwizigulane ezanyangwa ngeeseli zeCAR-T eziqhutywa yi-MND xa kuthelekiswa nezo zanyangwa ngeeseli eziqhutywa yi-EF1A.
UGqr. Peihua Lu waseLu Daopei Hospital ubonakalise ithemba malunga nokuba le ndlela intsha inokwenzeka na, esithi, "Intsebenziswano yethu neHebei Senlang Biotechnology ivelise ulwazi olubalulekileyo malunga nokwenza ngcono unyango lweeseli zeCAR-T. Ngokulungisa umkhuthazi, sinokuphucula iprofayili yokhuseleko lonyango ngelixa sigcina ukusebenza kwalo. Eli linyathelo elibalulekileyo ekwenzeni unyango lweCAR-T lufikeleleke ngakumbi kwaye lunyamezeleke kwizigulana."
Olu phononongo luxhaswe zizibonelelo ezivela kwiNatural Science Foundation yePhondo laseHebei kunye neSebe leSayensi neTekhnoloji yePhondo laseHebei. Lugxininisa ukubaluleka kokukhethwa kwabakhuthazi ekuphuhlisweni konyango lweeseli zeCAR-T kwaye luvula iindlela ezintsha zokhuseleko nokusebenza ngakumbi. Unyango lomhlazas.